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recombinant human siglec  (R&D Systems)


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    Structured Review

    R&D Systems recombinant human siglec
    Recombinant Human Siglec, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/siglec+3+fc+chimera+protein/Recombinant+Human+Siglec-3%2FCD33+Fc+Chimera+Protein%2C+CF/bio_rxiv__2025__07__11__664404-107-108-114
    Average 93 stars, based on 10 article reviews
    recombinant human siglec - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Cell surface ribonucleoproteins cluster with heparan sulfate to regulate growth factor signaling
    Article Snippet: MOLM-13 cells were counted and then blocked as per the manufacturer’s protocol with Human TruStain FcX (Fc block, BioLegend) for 15 minutes on ice before labeling. .. For Siglecs staining in live cells, 1 μg/mL of recombinant human IgG1 Fc (R&D Systems), Siglec-1 Fc chimera protein (R&D Systems), Siglec-2 Fc chimera protein (R&D Systems), Siglec-3 Fc chimera protein (R&D Systems), Siglec-4 Fc chimera protein (R&D Systems), Siglec-5 Fc chimera protein (R&D Systems), Siglec-6 Fc chimera protein (R&D Systems), Siglec-7 Fc chimera protein (R&D Systems), Siglec-8 Fc chimera protein (R&D Systems), Siglec-9 Fc chimera protein (R&D Systems), Siglec-10 Fc chimera protein (R&D Systems), Siglec-11 Fc chimera protein (R&D Systems), Siglec-14 Fc chimera protein (R&D Systems), and Siglec-15 Fc chimera protein (R&D Systems) were precomplexed with 0.5 μg/mL of donkey anti-human IgG AF647 (ImmunoResearch) secondary antibody in FACS buffer (0.5% BSA (Sigma) in 1x PBS) for 45 minutes on ice. .. For 9D5 and 10E4 staining, 2.5 μg/mL of 9D5 (Absolute Antibody), 1 μg/mL of anti-heparan sulfate 10E4 (amsbio), and anti-VEGF (R&D Systems) were precomplexed with 1.25 μg/mL of goat anti-Rabbit AF647 secondary antibody (ThermoFisher Scientific), 0.5 μg/mL of goat anti-Mouse AF647 secondary antibody (ThermoFisher Scientific), or donkey anti-Goat IgG AF647 (ThermoFisher Scientific) secondary antibodies in FACS buffer for 45 minutes on ice, separately.

    Recombinant:

    Article Title: Cell surface ribonucleoproteins cluster with heparan sulfate to regulate growth factor signaling
    Article Snippet: MOLM-13 cells were counted and then blocked as per the manufacturer’s protocol with Human TruStain FcX (Fc block, BioLegend) for 15 minutes on ice before labeling. .. For Siglecs staining in live cells, 1 μg/mL of recombinant human IgG1 Fc (R&D Systems), Siglec-1 Fc chimera protein (R&D Systems), Siglec-2 Fc chimera protein (R&D Systems), Siglec-3 Fc chimera protein (R&D Systems), Siglec-4 Fc chimera protein (R&D Systems), Siglec-5 Fc chimera protein (R&D Systems), Siglec-6 Fc chimera protein (R&D Systems), Siglec-7 Fc chimera protein (R&D Systems), Siglec-8 Fc chimera protein (R&D Systems), Siglec-9 Fc chimera protein (R&D Systems), Siglec-10 Fc chimera protein (R&D Systems), Siglec-11 Fc chimera protein (R&D Systems), Siglec-14 Fc chimera protein (R&D Systems), and Siglec-15 Fc chimera protein (R&D Systems) were precomplexed with 0.5 μg/mL of donkey anti-human IgG AF647 (ImmunoResearch) secondary antibody in FACS buffer (0.5% BSA (Sigma) in 1x PBS) for 45 minutes on ice. .. For 9D5 and 10E4 staining, 2.5 μg/mL of 9D5 (Absolute Antibody), 1 μg/mL of anti-heparan sulfate 10E4 (amsbio), and anti-VEGF (R&D Systems) were precomplexed with 1.25 μg/mL of goat anti-Rabbit AF647 secondary antibody (ThermoFisher Scientific), 0.5 μg/mL of goat anti-Mouse AF647 secondary antibody (ThermoFisher Scientific), or donkey anti-Goat IgG AF647 (ThermoFisher Scientific) secondary antibodies in FACS buffer for 45 minutes on ice, separately.

    FACS:

    Article Title: Cell surface ribonucleoproteins cluster with heparan sulfate to regulate growth factor signaling
    Article Snippet: MOLM-13 cells were counted and then blocked as per the manufacturer’s protocol with Human TruStain FcX (Fc block, BioLegend) for 15 minutes on ice before labeling. .. For Siglecs staining in live cells, 1 μg/mL of recombinant human IgG1 Fc (R&D Systems), Siglec-1 Fc chimera protein (R&D Systems), Siglec-2 Fc chimera protein (R&D Systems), Siglec-3 Fc chimera protein (R&D Systems), Siglec-4 Fc chimera protein (R&D Systems), Siglec-5 Fc chimera protein (R&D Systems), Siglec-6 Fc chimera protein (R&D Systems), Siglec-7 Fc chimera protein (R&D Systems), Siglec-8 Fc chimera protein (R&D Systems), Siglec-9 Fc chimera protein (R&D Systems), Siglec-10 Fc chimera protein (R&D Systems), Siglec-11 Fc chimera protein (R&D Systems), Siglec-14 Fc chimera protein (R&D Systems), and Siglec-15 Fc chimera protein (R&D Systems) were precomplexed with 0.5 μg/mL of donkey anti-human IgG AF647 (ImmunoResearch) secondary antibody in FACS buffer (0.5% BSA (Sigma) in 1x PBS) for 45 minutes on ice. .. For 9D5 and 10E4 staining, 2.5 μg/mL of 9D5 (Absolute Antibody), 1 μg/mL of anti-heparan sulfate 10E4 (amsbio), and anti-VEGF (R&D Systems) were precomplexed with 1.25 μg/mL of goat anti-Rabbit AF647 secondary antibody (ThermoFisher Scientific), 0.5 μg/mL of goat anti-Mouse AF647 secondary antibody (ThermoFisher Scientific), or donkey anti-Goat IgG AF647 (ThermoFisher Scientific) secondary antibodies in FACS buffer for 45 minutes on ice, separately.



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